M13ko7 helper phage sequence
http://www.nanobody-biolab.com/upload/file/2024-03-27/6372092434280348534756052.pdf WebGeneral description. The phage is an M13mp1 derivative containing a modified gene 11 [G changed to T at position 6,125, giving a met to ile change at codon 40 of the gene 11 protein (1)], an insertion of the origin of replication of p15A and the kanamycin resistance gene from Tn903 at the Ava I site (position 5,825).
M13ko7 helper phage sequence
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Web13 apr. 2024 · The Sanger sequencing result confirmed the presence of the gene in the correct orientation within the construct. Infection of pAK8-P1-bearing TG1 bacteria with M13KO7 helper phages, followed by a PEG-NaCl precipitation step, resulted in the production of ~10 12 pfu/mL of recombinant phage.
Webing of phage to antigen comparing to M13KO7 helper phage. The predominance of this phage is its utility in stoichiometric situations, when single phage could hardly locate the desired antigen.12 Moreover, hybrid phage system enables displaying large pro-teins with all five M13 coat proteins as N-terminal fusions with Web7 apr. 2024 · Porcine-scFv phage display library. The amplicons of porcine vh (450 bp) and vl (350 bp) are shown in Figure 1A and 1B, respectively. SOE-PCR was used to link the vh and vl sequences randomly via a (Gly4Ser1) 3 linker (Supplementary Table S1). The pscfv amplicon (~800 bp) is shown in Figure 1C. The pscfv repertoire was inserted into …
Web17 dec. 2024 · For 500 mL, a total 2.5 cells × 10 11 cells are expected and 5×10 12 pfu of M13KO7 helper phage corresponds to and MOI of 20. d. Add kanamycin to a final concentration of 25 μg/mL and grow for 16–20 h at 37°C, 250 rpm. ... the corresponding monoclonal phage may be isolated by sequencing individual bacterial colonies by … WebM13KO7 helper phage is suitable for the infection of F+ or F’ E. coli bacteria containing a phagemid vector having an f1 or f1-like origin of replication. The g3p fusion protein is …
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WebM13KO7 is able to replicate in the absence of phagemid DNA. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid is packaged … food city west newport tennesseeWebM13KO7 Helper Phage is used in conjunction with the phagemid of choice. In the presence of a phagemid bearing a wild-type M13 or f1 origin, single-stranded phagemid DNA is … el.appendchild this._elcanvasWebHowever, if you are only using KM13 as a helper then you can substitute M13KO7, the major difference I believe is that KM13 has a trypsin site in the tail protein, so any trypsin … food city winchester tn openingWeb6 apr. 2024 · Phage amplification, tittering and phage display technology The antibody library was added to 2 YT liquid medium with 2% d -glucose and 100 μg/mL ampicillin, incubated at 37 °C, 200 rpm for 1–2 h; When OD 600 of the culture reached 0.4–0.5, M13KO7 helper phage was added according to the ratio of phage: TG1 = 20:1, and … ela phillyWeb1.80 ml. $177.00. $159.30. *On-line ordering is for Canadian customers only. Web pricing is applicable only to orders placed online at www.neb.ca. M13KO7 Helper Phage is used … elap montgomery collegehttp://www.jinpanlab.cn/archives/date/2024/03/24/page/13 elaphurus davidianus iucn red listWeb16 mai 2024 · The same amount of M13KO7 helper phage was used as a negative control (denoted as M13wt). The bound phages were detected with anti-M13 antibody conjugated with horseradish peroxidase (HRP). The binding signal was analyzed at absorbance (A) 405. elap proficiency testing