WebAbstract. A new human megakaryocytic cell line (Dami) has been established from the blood of a patient with megakaryoblastic leukemia. The Dami cells grow primarily in … Web20 aug. 2024 · Megakaryoblastic leukemia cell line (MEG-01) was purchased from ATCC (Manassas, VA, U.S.A.), cultured in Roswell Park Memorial Institute (RPMI) 1640 medium (Gibco; Thermo Fisher Scientific, Waltham, MA, U.S.A.) supplemented with 10% fetal bovine serum (FBS; Merck Millipore, Darmstadt, Germany) at 37°C in a humidified atmosphere …
Characterization of a new megakaryocytic cell line: the …
WebTranscription factors regulating the process of megakaryocyte development remain largely unclarified. To clarify them further, we used a human megakaryoblastic cell line, Meg-J, which showed prominant polyploidization and augmented platelet glycoprotein (GP) Ib expression after incubation with thrombopoietin (TPO, c-mpl ligand) and K252a (an … Web29 sep. 2024 · First, C2C12 cells (2 × 10 6) were incubated for 15 min at 4 °C with digitonin (Sigma, D5628) (8 μM/million cells), prepared in MiRO5 buffer to the permeabilize cells. Non-phosphorylating respiration was induced by adding the complex I-linked substrate 2 mM malate (Sigma, M1000) and 10 mM glutamate (Sigma, G1626), followed by 2.5 mM ADP … fashionable fireplaces
Valproic acid and all trans retinoic acid differentially induce ...
Web10 okt. 2013 · acute megakaryocytic leukemias. View large Download PPT. A 46-year-old woman presented with diffuse bone pain. She had a history of acute myeloid leukemia … WebA megakaryoblastic cell line, termed T-33, was established from the peripheral blood of a patient with Philadelphia chromosome-positive chronic myelogenous leukemia in megakaryoblastic crisis. T-33 cells have been maintained in RPMI 1640 medium containing 10% fetal calf serum in a single cell suspension with a doubling time of 24-36 … WebAcute megakaryoblastic leukemia. Patients with acute megakaryoblastic leukemia usually have cytopenias, although some may have thrombocytosis. Dysplastic features are often present in all cell lines. Diagnosis requires the presence of at least 20% blasts, of which at least 50% must be of megakaryocyte origin. fashionable fitness tracker 2017