Psicheck vector
WebSpecimen collection. The samples from 95 ovarian cancer patients were collected from 2012 to 2024. The diagnosis of all patients was confirmed by surgery and pathology results in the Shengjing Hospital of China Medical University, and all patients only received PTX-based chemotherapy after surgery, the written informed consent form was obtained prior … WebpsiCHEC-2 Vector is designed to provide a quantitative and rapid approach for optimization of RNA interference (RNAi). The vectors enable the monitoring of changes in expression of a target gene fused to the reporter gene.
Psicheck vector
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WebHere we demonstrate the use of the psiCHECK™-2 Vector as a biosensor for miRNA-21 (miR-21) in different cell lines and as a tool to test an miRNA target. Generating an miRNA … WebApr 10, 2024 · PFN2 DNA sequences including or lacking predicted miRNA-binding sequence were inserted into the psiCHECK-2 vector (C8021; Promega, Madison, WI, USA). Transfection of the purified plasmid vectors into HNSCC cells were performed using Lipofectamine 2000 (Invitrogen) at 50 ng/well. After 48 h of transfection, we conducted dual luciferase …
WebThe psiCheck-2 vector contains two luciferase genes, renilla as the experimental reporter while firefly the control reporter. Renilla is the experimental reporter as you can clone in regulatory ... WebNov 3, 2024 · psiCHECK™-2 Vector（ C8021 ） 最初是为验证 siRNA 靶标设计的。 该载体用海肾萤光素酶（hRluc）作为主报告基因； 萤火虫萤光素酶（hluc+）则作为内参 报告基因，用于进行归一化处理。 目的基因被克隆至海肾萤光素酶翻译终止密码子下游的多克隆位点 …
WebThe psiCHECK™-1 Vector and psiCHECK™-2 Vector provide a quantitative and rapid approach to optimize RNA interference (RNAi). The vectors enable monitoring of changes in expression of a target gene fused to the reporter gene. WebThe psiCHECK™-1 Vector and psiCHECK™-2 Vector provide a quantitative and rapid approach to optimize RNA interference (RNAi). The vectors enable monitoring of changes …
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WebThe psiCHECK™-1 Vector(a,b,c) (Cat.# C8011) and psiCHECK™-2 Vector(a,b,c) (Cat.# C8021) are designed to provide a quantitative and rapid approach for optimization of RNA … butte county conservation districtWebDec 23, 2016 · (A) Schematic representation of dual luciferase reporter plasmid psiCHECK-2 vector containing the 3′ UTR of Dlx5 inserted downstream of Renilla luciferase gene. Four types of dual luciferase reporter plasmids were constructed, one wild type and three types of mutations were constructed within the seed region of the miR-203 and miR-320b ... cdkeys psn ushttp://www.honorgene.com/uploads/files/202404/5ea518d17dd8f.pdf butte county county jobsWebpsiCHECK-1 and psiCHECK-2 Vectors enable monitoring of changes in expression of a target gene fused to a reporter gene. They are used for optimizing RNA interference … butte county court addressWebThe psiCHECK™Vectors provide a quantitative and rapid approach for the optimization of RNAinterference (RNAi). The vectors enable changes in expression of a target gene to be determined by monitoring the activity of a fused reporter gene. In the psiCHECK™Vectors, Renillaluciferase is used as the reporter gene. cdkeys ps plus code not workingWebDual-luciferase reporter assay The psiCHECK-2 vector ( Promega, Cat # C8021) was used to construct plasmids for dual-luciferase reporter assay. RNF146 3′UTR or its mutant was cloned October 12, 2024 Zhaowei Wang, Xiaoling Xia, Jiaqi Li, Tatsushi Igaki cd keys psn subscriptionWebBy cloning the 3’UTRs of collagen IV subunits into the psiCHECKTM-2 vector, we found that seven of the eight sites in the Col4a3–Col4a6 gene complementary to miR-29 were significantly repressed by miR-29a, b (except for the … butte county courthouse south dakota